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Pirh2-overexpressing myeloma cell lines ARP-1-Pirh2, ARK-Pirh2, LP-1-Pirh2 and their corresponding controls were established as described earlier.
An integrated pig-biogas-vegetable greenhouse system (PBVGS) was designed and studied in Laiwu, Shandong Province of North China from 2001 to 2002, where 20 groups of PBVGS and their corresponding controls were investigated.
The Pirh2-overexpressing myeloma cell lines were named ARP-1-Pirh2, ARK-Pirh2 and LP-1-Pirh2, and their corresponding controls were named ARP-1-ctl, ARK-ctl and LP-1-ctl.
The resultant cells infected with lentiviruses containing Pirh2-targeting sequencesuences were referred to as RPMI8226-shPirh2, OPM-2-shPirh2, and NCI-H929-shPirh2, and the cells infected with the corresponding controls were referred to as RPMI8226-ctl, OPM-2-ctl, aNCI-H929-ctlctl.
The corresponding controls were stained only with secondary antibodies.
The corresponding controls were either FITC-IgG2b (1∶1000) or PE-IgG1 (1∶1000).
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The difference in inhibitory effect at different doses between treated and corresponding controls was analyzed for statistical significance by performing Student's t test.
* P<0.05 and ** P<0.01 versus the corresponding controls are indicated.
After normalization, the difference between gene expression and corresponding controls was calculated (paired design).
The baseline characteristics of incident HCC cases and their corresponding controls are presented in Table 1.
Results shown to the left and below the dotted diagonals (experiments and their corresponding controls) are from 2001 or 2002.
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