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The corresponding control mice were administrated with an equivalent volume of vehicle.
Compared to corresponding control mice, diabetic mice showed increased levels of the pro-inflammatory cytokines TNF-α and IL-6.
All mice as well as the corresponding control mice were born, generated and housed at the animal facility of Robert Debré Hospital, Paris, France.
Kato et al. demonstrated that the expression miR-192 is increased in renal glomeruli obtained from mouse models of type 1 and type 2 diabetes relative to corresponding control mice [14].
Values are means ± SEM. * p < 0.05 versus corresponding control mice.
The accumulation of 4-HNE in both WT and FGF21KO diabetic mice elevated since the 2nd month after diabetes onset compared to the corresponding control mice.
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Neutralizing antibodies against IL-1α and IL-8 were purchased from Abcam (Cambridge, MA, USA) and the corresponding control anti-mouse IgG and anti-rabbit IgG were purchased from Santa Cruz.
Cells were incubated for 40 minutes at 4°C with FITC or PE conjugated antibody to CXCR4 (1∶50, Pharmingen), N-CAM (1∶200, Pharmingen), or PDGFαR (1∶50, Pharmingen) or the corresponding isotype control (mouse IgG isotype control, Pharmingen).
For each microsomic mouse detected (36 males and 38 females), the corresponding littermate control mouse was identified (37 males and 35 females).
Excisional (6 mm) wounds created on the back of these mice showed severe impairment in closure when compared to their corresponding control (heterozygous db/+) mice (data not shown).
Five groups of five Nod2−/− mice in the C57BL/6j genetic background as well as the corresponding Nod2+/+ control mice were orogastrically inoculated with 10-fold serial dilutions of the Y. pseudotuberculosis strain YPIII (pIB102) [17] (supplementary Figure S1a).
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