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Coomassie blue-stained gels are shown below each corresponding blot.
The corresponding blot, revealed with an anti-V5 antibody, indicated that Akirin associated with BAP60 (Supplementary Fig S2D).
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c Changes in VEGF isoforms were measured using densitometry in the corresponding blots.
The corresponding blots were blocked in a TGG solution (50 mM Tris pH 7.4, 100 mM NaCl, 1 M glucose, 10% glycerol, 0.5% Tween-20) with 1% skim milk and 3% BSA, for 1 h at 4°C.
Due to the fact that total MEK1/2 changed during the treatments, in the experiments depicted in Fig. 3, we also evaluated the contents of an acyl-CoA thioesterase (Acot2), 39 kDa subunit of the NADH-cytochrome c reductase (complex I) and β-tubulin in the corresponding blots.
LC3-II detection visualized in the presence or absence of pepstatin A and E64d, effectively increases with age in lupus-prone mice, both at steady-state and under PMA/ionomycin stimulation (as quantified for corresponding blots in Fig. 4C and D).
Figure 3A shows the mean liver ENPP1 protein abundance for all 13 individuals who had liver protein extracted at RYGB surgery and on average 17 months later, while Figure 3B is the corresponding western blot (see Additional file 1 - Uncropped Western Blots - Figures 2 (ENPP1) and 4 (Actin) for original uncropped images).
Comparisons of the spot intensities between the silver-stained gel and the corresponding ligand blot show that glutamate dehydrogenase (Q5KL32, indicated by * in Fig. 6B) exhibited the highest relative affinity for plasminogen among the reactive spots, even though it was not detectable in the silver-stained 2D gel.
Figure 5 illustrates a separation of the 14 P.falciparum chromosomes and the corresponding Southern blot, probed with a DBL3X-derived var2csa-specific gene probe.
This finding was supported by corresponding western blot data.
The corresponding dot blot analysis for ssDNA confirms the presence of ssDNA in the peak fractions.
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