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Furthermore, we analyzed the proteome of prostates from 24 cancer patients and their corresponding benign tissue in 21 cases (Table 1) by 2D-DIGE with the pI range of 4.0 7.0 and molecular weight range between 10 kDa and 120 kDa.
For this, we generated low-coverage whole genome sequencing profiles for the two tumor foci and the corresponding benign tissue from each patient.
However, upregulation of ANXA1 mRNA was observed in 3 of 8 (37.5%) cases, which might be attributable to an abundant accumulation of cells in the tumor tissue compared with the corresponding benign tissue.
The majority of the breast cancer specimens (15 out of 21), ovarian cancer specimens (6 out of 8) and malignant melanoma specimens (5 out of 6) were found to express TRAP, whereas the corresponding benign tissue showed markedly weaker expression or none at all.
For somatic SNV detection from two biopsies of the same prostate cancer tumor the Phred-scale cutoff was required to be greater or equal to 20 and the SNV was required to be found in both tumor foci in at least 4 reads but not in the corresponding benign tissue with a minimal coverage of 10 fold.
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For immunohistochemistry paraffin sections of tissue microarrays comprising prostate cancer and corresponding benign prostatic tissue samples were treated with xylene and ethanol.
There was no significant difference to corresponding benign prostatic tissue.
A comparison with corresponding benign prostatic tissue showed that CD57 expression in the prostate is not a tumor-specific phenomenon.
Only seven cases (1%) showed a significant stronger CD57 expression in the tumor, whereas 70 cases (9%) displayed a weaker expression by more than one scoring point as compared to the corresponding benign prostatic tissue.
Moreover, the expression level of MIC-1 was also enhanced in 72% of prostatic adenocarcinomas analysed relative to their corresponding adjacent benign tissues from 32 PC patients (Gleason scores=7 9; mean of composite scores: 3.9±0.4 vs 1.5±0.3; P⩽0.0001), respectively.
Immunostaining was carried out on AccuMax array tissue sections (Petagen, Inc., Shinchon-dong, Seoul, Korea) containing 8 normal prostatic tissue specimens of biopsy as well as AccuMax array containing 32 pairs of tissue sections from patients with primary prostatic adenocarcinoma (Gleason scores 7 9) with their corresponding adjacent benign tissues from the same patients.
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