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These samples were subjected to the corresponding assays in order to determine the maximum additive percentage to be added according to standards.
Subsequently, cells were washed and used for the corresponding assays.
The corresponding assays monitor the Wnt and the hedgehog pathway.
The 48 genes and corresponding assays are listed in Additional file 1.
On day 5, cells were processed for corresponding assays and the efficiency of protein depletion was assessed by western blotting.
Brucella melitensis and Leishmania infantum isolated from human patients were used as positive controls for the corresponding assays.
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WBs analyses with antibodies against endogenous (endog). proteins in co-silenced HCC cells used for the corresponding assay.
As a positive control, an oligonucleotide containing U, 5 hmU or T instead of C, 5 hmC and 5 mC, respectively, (Supplementary Table S5) was used in the corresponding assay.
The supernatant (50 μL) was used to detect catalase (CAT), superoxide dismutase (SOD), ascorbate peroxidase (APX) and glutathione reductase (GR) activity by using the corresponding assay kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China).
Beads were washed once in corresponding assay buffer.
Since the corresponding assay could be routinely and easily run by any microbiology laboratory, typing data from different groups could be merged and compared without the need to exchange live strains.
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