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Next we created a single maximum likelihood (ML) tree topology and corresponding alignment for each of the three genomes (nuclear, plastid, and mitochondrial) for each of the 12 sister pairs (36 topologies and alignments in total).
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This is a real-time preprocessing phase for detecting the possible presence and the corresponding alignment of the string in the data flow.
The corresponding alignment positions for the three species that lack rRNA gene data were assigned with gaps.
The probability of finding in random networks two nodes with the same or higher interaction overlap as a given alignment is estimated, and serves as a p-value for the corresponding alignment [See Additional file 1 for details.] We use an iterative algorithm as described in [ 7] to find the high-scoring graph alignments.
As a compromise, we choose to split the alignment according to some pre-established cut-points, in submatrices that are small enough to fit into memory, and that are recomputed only once for retrieving the corresponding alignment fragments.
The ROI chosen for each test case, and the corresponding alignment search space are presented first.
The computed similarity scores for each conformer pair were stored with the translation/rotation matrix that yields the corresponding alignment.
Alignments of the top 20 hits are extracted from the corresponding alignment dataset.
It returns the corresponding alignment.
The corresponding alignment is then constructed by, first, tracing back the maximum location in each matrix and, then, tracing back alignments for the 5′ and 3′ ends (i.e. alignment is inferred from each matrix) and combining them (Fig. 3C).
The HOMSTRAD/Pfam benchmark comprises of Pfam entries containing up to 10,000 sequences, which had a corresponding structural alignment for two or more of the sequences in HOMSTRAD.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com