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While the expression of PCNA, Bcl-2, p53 and ER was significantly increased, expression of cytokeratin was significantly lower in both human as well as canine mammary tumours compared to corresponding adjacent tissues.
The clinical association between miRNAs and hTERT was verified within 58 pairs of gastric cancer tissues and corresponding adjacent tissues.
hTERT-positive gastric cancer tissues and hTERT-negative corresponding adjacent tissues were collected from the same patient undergoing surgical operations.
The paired-samples t-test was used to assay the differences in the miRNA expression levels between cancer tissues and corresponding adjacent tissues.
Genomic DNA (gDNA) from NSCLC tissues and corresponding adjacent tissues was isolated using the DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions.
To further investigate the expression of CD133 protein in carcinomas and the corresponding adjacent tissues, we performed IHC analysis on primary patient NSCLC specimens.
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Unlike endometrioid cancer and its tumour-adjacent tissues, these cluster vectors are near-identical, representing similarity between non-endometrioid cancer and its corresponding adjacent tissue.
Among these 57 miRNAs, 14 expressed at lower level in the hTERT-positive gastric tumor tissue than that of hTERT-negative corresponding adjacent tissue.
Differential methylation was assessed by comparing the mean methylation level (b-value) of samples with the mean b-value of the corresponding adjacent tissue by using BeadStudio (San Diego, CA, USA) and Qlucore Omics Explorer 2.0 (Qlucore AB, Lund, Sweden) software.
We further analyzed Nestin mRNA levels from 28 paired NPC patient tissues and corresponding adjacent normal tissues and found that the average Nestin mRNA levels were 1.75-fold higher in NPC tissues than in adjacent normal tissues (P=1.06E−06).
8 colorectal cancer tissues and their corresponding adjacent normal tissues ere selected for microRNA microarray analysis from the 40 matched tissues pairs of CRC tissues and their adjacent normal colorectal mucosa tissues.
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