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Approximately 250 ng of DNA were digested with EcoRI and MseI restriction enzymes and ligated to corresponding adapters.
Digested fragments were ligated with corresponding adapters and then amplified using one-base selective primers (HindIII-A and MseI-T) according to standard AFLP protocol [ 22].
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Of course, to get these results you need a Draft N wireless router and corresponding adapter for all your devices on the network.
With the adapter board, we cannot only easily join the sensor node and test board together to form a test unit, (see Figure 13) but also support a variety of sensor nodes via its corresponding adapter with just one kind of standard test board.
Where the prosthetic is removable, a corresponding adapter is placed in the prosthetic so that the two pieces can be secured together.
Residual single-stranded cDNA, rich in tester-specific cDNA population, was specifically amplified by PCR with primers corresponding to the adapters.
We then used primers corresponding to the adapters (5′-GTTTAAGGCCTAGCTAGCAGAATC-3′ and 5′-GATTCTGCTAGCTAGGCCTT-3′) to amplify the microsatellite-enriched DNA.
Subsequent PCR amplifications, first with primers corresponding to both adapters then with nested primers, selectively amplified and enriched the differentially expressed cDNAs.
Perl scripts were used to remove microreads containing N's and those reads corresponding to Illumina adapter sequences from the chastity-filtered short read pool to create a cleaned read pool [ 45].
The double-stranded cDNA was digested with EcoRI and MseI and ligated to the corresponding EcoRI and MseI adapters.
DNA from each eluted sample was enriched by 18-cycle PCR using a high-fidelity polymerase and a single primer pair corresponding to the Illumina adapters ligated earlier.
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CEO of Professional Science Editing for Scientists @ prosciediting.com