As the total mass of aerated lung tissue did not change, these density modifications anatomically correspond to already open lung units that get more inflated in prone position (poorly aerated lung tissue which becomes well aerated).
Twenty of them correspond to already known regulators of the pathways after literature and Gene Ontology annotation searches, thus validating our approach.
Conversely, very few of the candidate sequences identified correspond to already annotated sequences for sizes between 10 and 30 amino acids.
They clearly correspond to already published environmental sequences from deep-sea Antarctic plankton (DH148-5-EKD18 [ 3]), from the Guaymas Basin hydrothermal vent (CS_R003 [ 7]), and from anoxic, marine sediments collected in Bolinas Tidal Flat (BOL1 cluster [ 5]).
Thirty regions correspond to already annotated non-coding RNA structures, including an highly abundant candidate (representing 0.4% of all aligned reads) that overlaps the U2 snRNA gene located on chromosome 5, and the 5S ribosomal RNA on chromosome 3, as previously noted for human [ 36].
The researchers could dismiss most of those variations — either they corresponded to already known conditions, or they occurred frequently enough in the general population to rule out their being the cause of a rare disease, or they were involved in biological processes that were unrelated to the patient's symptoms.
Overall, after validation, 47% of the tested loci (n = 44) turned out to be variable, and the majority of them (86%; n = 38) corresponded to already known CNVs, thus only 6 were novel.
It should be remembered, however, that 2010 corresponded to already the fourth year of pediatric HEN reimbursement/availability in Poland.
