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Further development of this technology could define a new platform 3D matrix, adaptable for a range of automated cell manufacturing, tissue modeling, and in vitro in vivo correlation assays, as desired.
To assess if cathepsin activity is affected by age (Additional file 3), we carried out correlation assays for age versus cathepsin S/B intensity amongst all groups (in upper graphs for serum and SF) and within each group (B, Table, Additional file 3).
Results from correlation assays were submitted to the U Mann-Whitney nonparametric comparing biofilm formation status and ica status, MP test, and CRA plate test.
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The main limitation of this study is that a small sample of real tissue was used for the correlation assay.
Pearson's coefficient correlation was used for expression correlation assay.
The significance of correlation with clinical traits was determined by Pearson's correlation assay with p <0.05.
Genomic data was retrieved from the GUDMAP database (Harding et al, 2011) and analysed using Pearson correlation assay across the normalized gene expression profiles.
According to our biological correlation assay, we found greater downregulation of VEGF and active MMP-9 in RKO clones treated with bevacizumab regimen than those without treatment.
For three subjects, we compared cytokine measurements for TNF-α obtained by the multiplex assay with measurements obtained using an enzyme-linked immunosorbent assay (Quantikine, R&D Systems, Minneapolis, MN) to validate the multiplex assay (correlation between assay measurements: R = 0.966; data not shown).
Statistical analysis of ELISA, IFA and Western blotting titers (from all volunteers and time-points measured) revealed a close correlation between all assays.
Overall, RT-PCR results validate the microarray data and demonstrate good correlation between two assays.
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