Sentence examples for correctness of assembly from inspiring English sources

To check the correctness of assembly 10 pair of primers were designed in one of the contigs and PCR reactions were performed.

Later a series of PCR, suggested on the basis of contig graph, were performed to join these contigs and check the correctness of assembly.

The choice of scaffold assembly method could be guided by the relative importance of size of scaffold and correctness of assembly.

We preferentially chose species for inclusion in GAGE-B for which a finished genome was available, to allow us to compute the correctness of assemblies.

In order to examine the correctness of the assembly process, we compared the SNPs derived from our assemblies to results from an earlier study from Rattei et al. [ 12], in which selected chromosomal regions of the same Cpn isolates have already been sequenced.

A region of 403,443 bp (preliminary experiment; see Text S1) was used to monitor the correctness of the assembly.

It should be noted that the most popular metrics for evaluating an assembly (e.g., contig size and N50), emphasize only size, poorly capturing the contig quality as they do not contain all the information needed to judge the correctness of the assembly.

This could help in evaluating length distribution of the sampled fragments and correctness of their assembly.

We then assessed the correctness of the assembly by comparing it with published paralogous genes.

Accuracy is a measure of the correctness of the assembly and is estimated by aligning each contig to the reference genome.

Comparison between the NcoI digest of the whole-genome optical map and in silico-generated physical maps of contigs showed that the largest contig (3 Mb) was mapped onto the chromosome, confirming the correctness of the assembly (Fig.  1A).