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Further, even in the era of high throughout sequencing, there are missing gaps caused by technical inability to correctly sequence tandem repeats and to accurately determine their copy number and chromosome position.
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Turning the ideas, now correctly sequenced, into composed episodes is arduous work, he confessed.
It is important to underline that the methodological approach proposed in this paper innovates in the joint, coordinated and correctly sequenced use of well-accepted and developed techniques that have gained a substantial consensus among researchers and practitioners.
A certain percentage (∼15%) of the homopolymer-containing amplicons, will not be correctly sequenced by 454.
However, due to the massive capability of this approach, enough molecules will still be correctly sequenced and represented in the final dataset.
For example let x be the probability that the base at position i is correctly sequenced.
Using this method, we have constructed 442 shRNA expression vectors, most of which were correctly sequenced.
In particular, for ETD spectra, UniNovo reported significantly more correctly sequenced spectra than PEAKS.
When N = 1, UniNovo and PepNovo+ found about the same number of correctly sequenced spectra in CID2 and CIDL2 datasets, but UniNovo found about 35% more correctly sequenced spectra than PepNovo+ in CIDA2 dataset.
The results on HCD spectra also demonstrate that UniNovo finds the largest number of correctly sequenced spectra in general.
UniNovo found the largest number of correctly sequenced spectra among all the tested tools in most datasets.
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