Two-way ANOVA comparing the relative phopho-MET (Tyr1349) band intensities of cells treated with vehicle, PLX4720, or PD184352 in the presence or absence of HGF. A. Planned comparisons with the Bonferroni correction: Cells treated in the absence of HGF and treated with vehicle, PLX4720, or PD184352 compared to cells treated in the presence of HGF and treated with vehicle, PLX4720, or PD184352.
To test the efficiency of error correction, cells carrying CEN4 (CEN4-GFP) and SPB (SPC42-tdTomato) markers together with CDC20 under control of a methionine-repressible promoter (pMET-CDC20) were arrested in G1 using alpha factor and then loaded onto the microfluidic chamber.
We focus on one such compound, BRD6897, and demonstrate through secondary assays that it increases the cellular content of mitochondria as evidenced by fluorescence microscopy, mitochondrial protein content, and respiration, even after rigorous correction for cell size, cell volume, or total protein content.
After correction for cell viability, differences between control and DPI-treated cells remained significant.
No correction for cell shrinkage was performed here as this feature is expected to affect the studied cell populations to a similar degree.
Nevertheless, for complex solid tissue samples, the correction for cell composition is not straightforward, as it would require the isolations and profiling of individual cell types.
Further, the volumetric nature of SWIFT also allows fast in vivo imaging, motion correction, and cell identification throughout curved subcortical structures like the dorsal hippocampus, where cellular-resolution dynamics spanning hippocampal subfields can be simultaneously observed during a virtual context learning task in a behaving animal.
Correction to: Cell Death and Disease (2014) 5, e1412.
The technique allows for the correction of cell heterogeneity bias through transformation of the data in two ways, depending on the platform used.
Correction to: Cell Death and Disease (2013) 4, e788; doi: 10.1038/cddis.2013.321; published online 5 September 2013 Since the publication of this article, the authors have noted errors in the abstract section.
