Sentence examples for correcting mutant from inspiring English sources

Furthermore, subtle differences in drug action were detected in correcting mutant channel activity that, together with both the known genetic background and age of the patient, contribute to the distinct therapeutic responses observed clinically.

Recent advancement in technologies such as TALEN and CRISPR/Cas9 facilitates precise genetic engineering for generating mutant or correcting mutant cells.

We have designed strategies to(1) cut and destroy "poison pill" dominant mutations and (2) correct mutant alleles in recessive disorders.

Various novel approaches that correct mutant CFTR function [ 1], or activate alternate electrolyte secretory mechanisms are under investigation [ 2].

Second, using our CRISPR S1mplex and ArrayEdit technologies, we demonstrate that gene correction of both mutant alleles within an autosomal recessive case of Pompe disease rescues the disease phenotype more effectively than correcting either mutant allele.

Approximately two-thirds of the colonies picked were the correct mutant.

The correct mutant plasmid was then transformed into E. coli BL21 (DE3) for expression.

After fixation the presence of the correct mutant gene was confirmed in each case by PCR rescue and sequencing.

Ultimately, great care must be taken by the protein engineer to ensure that any observed improvement in protein function is correlated with the correct mutant gene sequence.

In both E. coli and S. enterica, it was possible to identify a correct mutant by screening fewer than a dozen colonies when the selection cassette contained a double terminator element, whereas it was necessary to screen forty colonies to find a correct mutant when the selection cassette contained a single terminator element (Table  3).

Here we report the first study using base editor (BE) system to correct disease mutant in human embryos.