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This meant we could compare total prevalence trends including all diagnosed cases to corrected total prevalence trends.
Image J was then used to measure the corrected total cell fluorescence (CTCF) of all cells with an area between 400 800 square pixels (~ 30 cells per genotype and construct).
The area and integrated density of the relevant channel for each cell was measured, and CTCF (corrected total cell fluorescence) was calculated as Integrated Density−(Area of selected cell × Mean fluorescence of background readings).
corrected total cell fluorescence.
The corrected total cell fluorescence (CTCF) was then determined by subtracting the background from the integrated density (CTCF = ID – background).
b The intensity of staining obtained with anti-VCAM-1 antibody was quantified as corrected total cell fluorescence (CTCF) (mean ± SEM).
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The corrected total-item correlation at baseline ranged from 0.32 0.87 in different domains.
Seven items had corrected total-item correlations of <0.2 and were removed, increasing the Cronbach's alpha of the 34-item MLCDP to 0.85 (Appendix 1).
The corrected total-item correlation, as an indicator of item specificity, ranged between 0.55 0.75, with a trivial floor score of 0.4% and a ceiling effect of 8.7%.
Cronbach's alpha was 0.87; the corrected total-item correlation ranged between 0.55 0.75, with trivial 'floor' score and low 'ceiling' effect.
The result showed that all four domains met the criterion for item convergence: each subscale had a significantly higher average item-corrected total correlation for corresponding domains as compared to non-corresponding domains.
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