All spectra were corrected for solvent background.
The maximal induction of positive control (corrected for solvent control, 0.1 0.2% ethanol) was set as 100%, and the responses of other chemicals were converted to a percentage of the maximum level.
In this procedure, the experimental scattering patterns were corrected for solvent, container and instrument background scatterings, X-ray polarization, sample absorption and Compton scattering to yield the total scattering for the solute, I q).
The acquired spectra were corrected for minor solvent contributions by subtracting the reference spectrum of the buffer.
CD spectra were corrected for the solvent CD signal recorded from 20 65 °C.
We report free energies derived from gas-phase optimisations with the BP86 functional, corrected for chloroform solvent (PCM method) and dispersion effects (i.e., at the BP86-D3(CHClevelevelevel
When necessary, spectra were corrected for the solvent background and water vapor contributions in an interactive manner using the Nicolet OMNIC 3.1 software to obtain the protein's vibration spectra.
Spectra were corrected for buffer and solvent effects, and the spectral result was used to determine the percent composition of α-helices, β-strands, turns, and unordered structures with CONTIN of the CDPro software package (http://lamar.colostate.edu/∼sreeram/CDPro).edu/∼sreeram/CDPro
The spectra were corrected for background (buffer, solvent, and each protein individually), and the maximal intensities were measured using Vinci version 1.5 (ISS Inc .. To determine dye effects, emission spectra (660 700 nm) of 25 nM Cy5-labeled hPPARα titrated with increasing concentrations of unlabeled hLXRα were recorded upon excitation at 650 nm.
where DsFfAwAsρr, and ρs are deswollen weight of the sample, fraction insoluble, sample weight, weight of the absorbed solvent corrected for swelling increment, density of rubber, and density of solvent, respectively.
The signal from a blank flowcell, (which had been activated and blocked as above, but to which no peptide had been immobilized) was subtracted from all SPR measurements to correct for bulk solvent effects.
