The protein extract was supplemented with 0.1 parts v/w dithiothreitol (Biorad, Munich, Germany), 0.1 parts v/w of ampholyte mixture Servalyte pH 2 4 (Serva, Heidelberg, Germany), corrected by the amount of urea added (correction factor = sample weight prior to addition of urea/sample weight after addition of urea), and stored at −80°C.
The protein extract was supplied with 70 mM dithiothreitol (Biorad, Munich, Germany), 2% v/w of ampholyte mixture Servalyte pH 2 4 (Serva, Heidelberg, Germany), corrected by the amount of urea added (correction factor = sample weight prior to addition of urea/sample weight after addition of urea), and stored at −80°C.
In any case, this number has to be corrected by the amount of N2O, which would be released by the biomass anyway, even if it had not passed through the animal stomach.
(8)To eliminate the effect of reaction that occurs during the heating time, the initial amount of aluminum (Al0) was corrected by the amount of aluminum after the shortest reaction time.
To avoid double counting, background economic data need to be corrected by the amount of disaggregated production happening in the foreground using cobalt price information and inputs to cobalt production shall also be taken out from the background.
The amount of each PCB congener in samples was corrected by the amount of that analyte in the procedural blank associated with the analytical batch.
We reasoned that if we corrected by the amount of diversity on a scaffold we should be able to better differentiate X-linked from autosomal scaffolds.
Thus, to compare the activity of both isoforms, the luciferase activity needed to be corrected by the amount of EDARADD protein detected in the lysates.
The difference between arbitrary fluorescence units recorded with or without 40 μM of the proteasome inhibitor MG132 (Affiniti Research Projects Limited, Exeter, Devon, UK) or 100 μM of the TPPII inhibitor AAF-chloromethylketone (Sigma-Aldrich, St . Louis MO, USA) in the reaction medium was calculated, and the final data were corrected by the amount of protein in the reaction.
The relative amount of each target in each sample was then corrected by dividing its amount by the corresponding normalization factor.
