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The life-cycle of a partial differential equation (PDE) solver is often characterized by three development phases: the development of a stable numerical discretization; development of a correct (verified) implementation; and the optimization of the implementation for different computer architectures.
"To fix this, give correct, verified information to the organizations and churches that are on the ground with these people.
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We know this new story is absolutely correct and verified, as it came directly from USC's new sports information director: Jon Lovitz.
Resulting products were sequenced to verified correct product amplification.
HAB verified, and corrected where necessary, all transcripts against the recordings.
S. pombe deletion mutant strains, including fkh1Δ, tco89Δ and tor1Δ deletion mutants, were obtained from the Bioneer version 2.0 (Bioneer Corporation, Daejeon, Korea) library and PCR-verified for correct deletions at both junctions.
We measured the percentage of queries with a split alignment that verified the correct SV breakpoint (within 5 bases) and the total number of verified breakpoints (Fig. 3A).
The ldtMt2 coding sequence was fully sequenced (Genewiz), compared to the published sequence in GenBank (http://www.ncbi.nlm.nih.gov/Genbank) and a verified clone with the correct sequence was transferred into the mycobacterial – E. coli Gateway shuttle vector pGS400H [46].
T-tests verified significant (p<0.05; FDR corrected, ref. [45]) per-point differences between the absolute profiles (non-neutral vs. neutral).
The correct construct (designated pAZ93) was PCR-verified and sequenced to ensure intact ORF.
The resulting PCR products were cloned into pGEM-T (Promega), sequence-verified, and subcloned into the correct pBDT3C and pGADT3C vectors.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com