The assays described in this report are designed specifically to identify compounds which result in the reactivation and correct trafficking of misfolded gonadotropin releasing hormone receptor and vasopressin type 2 receptor mutants, rather than those which act as agonists directly.
Loss of the helix-capping interaction in R165W may affect the correct trafficking of this receptor, providing a possible mechanism for the observed malfunction of this mutant.
This was associated with increased cone response and survival, suggesting that visual chromophore may act as a chaperone to improve sorting and correct trafficking of these proteins [16] [17], [49].
In fact, residues surrounding the PEXEL motif were found to be important in correct trafficking or folding of exported proteins [9], [10], and a recent study suggests that the short pentameric core motif alone is insufficient to cause protein traffic across the PV membrane [11].
We speculate that the dodecameric structure of the SV40TAg:p53 complex [28] may be incompatible with the topology of the LOC and CAT fusions or that the high molecular weight complex interferes with correct trafficking of the Och1p fusion proteins, a phenomenon that was observed when another large oligomeric complex was ectopically localized to the secretory pathway [29].
Correct trafficking of lysosomal enzymes requires posttranslational modifications [ 113].
As neither C-terminally truncated wild-type receptor localized to the cell surface, we concluded that the truncation also prevented correct trafficking of S1p2 receptors.
A previous study demonstrated a synthetic lethal interaction between chs6Δ and myo1Δ [ 37] suggesting that correct trafficking of Chs3p was important for survival in myo1Δ strains.
These data therefore support the theory that correct sphingolipid function is required for the correct trafficking of mCD8-EGFP into the class IV dendrite arbour and that a secretory deficit is present in neurons expressing d Spt1 C129W.
In relation to the vacuole's lytic function, VAM3 (YOR106W) encoding the syntaxin-like protein Vam3p (a vacuolar t-SNARE) is responsible for the correct trafficking and processing of proteinases A, B and carboxypeptidase Y and maturation of alkaline phosphatase [ 73].
