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The number of positive cells was corrected to the volume of the ROIs.
The average of all positive cells was corrected to the volume of the ROIs.
Preprocessing included spatial normalization to the Montreal Neurological Institute MNII) template, segmentation into the different tissue classes (grey matter, white matter, CSF), and modulation to correct for volume changes due to normalization.
This modulation step was performed to correct for volume change in the non-linear normalization.
Deoxy-guanosine was added as an internal standard to correct for volume changes during sample preparation and injection.
The images were modulated to correct for volume change induced by the normalization process.
Quantification was performed using external standards, with the polychlorinated biphenyl (PCB -142 injection standard used to correct for volume.
On both instruments, quantification was performed by external standards using PCB-142 as an injection standard to correct for volume.
Quantification was performed by using external standards, with the polychlorinated biphenyl congener 142 (PCB-142) injection standard to correct for volume.
The same volume of saline was injected in the hindlimbs (both operated and contralateral nonoperated limbs) of the other groups to correct for volume discrepancies during subsequent volumetric analyses.
Therefore, as an additional check of the robustness of our findings, we used fluorescent beads with diameters similar to those of contact zones measured with EM (Jones and Calverley 1991), to correct for the volume overestimation attributable to fluorescence imaging.
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