Sentence examples for correct the assembly from inspiring English sources
Additional discordances were derived from the sequences of the BACs/PACs, rather than from the pseudomolecule construction; no attempt was made to correct the assembly of the BAC/PAC sequences to match the optical maps.
We used the Roche 454 de novo assembly to map the SOLiD reads, identify errors and then correct the assembly.
After the release 1, an intensive collective work took place in order to improve sequence quality and annotation, fill in the gaps, and correct the assembly.
For SNP typing of bacterial strains, the sequence quality of the assembly is very important and there are several strategies to correct the assembly for sequencing errors, including the detection of frameshifts by comparative genomics, and the correction of SNPs in an assembly using Illumina reads [ 32, 33].
These two findings highlight the importance of the current need to use both PacBio and Illumina assemblies, instead of assembling only the PacBio data and then using the Illumina reads to correct the assembly, which appears to be the accepted practice [ 17, 18, 43].
Since this algorithm involves a splitting procedure of the contigs into restriction fragments, chimeric contigs were broken into non-chimeric parts thereby correcting the assembly errors mentioned above.
On the basis of our analyses, we corrected the assembly of the genome sequence of M. ap K-10 starting from MAP3759c to MAP0007, corresponding to 4,197,080 bp to 11,150 bp in the genome of M. ap K-10.
Our analysis indicated that the Illumina sequence always contained the correct number of bases in these homopolymeric stretches, and consequently it was used to correct the PacBio assembly.
Hi-C data were then used to correct the Bionano assemblies.
Once the known and putative misassemblies were broken, and the errors in the PacBio reads were corrected, the M_zebra_v0 assembly was ready to be improved using PBJelly.
Correcting the sequence assembly requires extensive additional mapping information to improve the reliability of the ordering of sequence scaffolds on chromosomes.
