The nature of the molecular cues involved in the correct targeting of the migrating precursors remains a question.
Correct targeting was confirmed by Southern Blot (Fig. 1C).
Correct targeting of hygromycin resistant Ring1b−/LoxHyg ES cell clones was confirmed by Southern blot analysis.
One of the six clones with correct targeting was designated NSB1.
The ES clones with correct targeting events were identified by Southern blot and PCR.
Correct targeting events and cre – loxP deletion events were confirmed by Southern blotting (Fig 1).
Resistant colonies were then analysed for correct targeting of the responder transgene to the ROSA26 locus.
This ensured that correct targeting had occurred at both ends of the integration.
Hence, the mutated aa are critical for both correct targeting and GDAP1-mediated fission.
Thus, like in other organisms, LdARL-1 must be N-myristoylated for its correct targeting to the TGN.
The selected ES clones were screened by Southern blotting with appropriate 5' and 3' probes to verify correct targeting.
