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The robot actions were defined as 12 movement directions in 3D space and the task of the Actor was to select the correct sequence of actions (based on M1 and NAcc neural activity) that navigated the robot to the target.
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The recombinant plasmids were sequenced to confirm the correct sequence of the inserted genes.
Sequence analyses confirmed the correct sequence of the different plasmids.
The correct sequence of all PCR products was confirmed by DNA sequencing.
The correct sequence of the insert was verified by DNA sequencing.
The correct sequence of the generated plasmid was confirmed by nucleotide sequence analysis.
The correct sequence of pCMV6-NEK2-GFP was verified by sequencing.
The correct sequence of the inserted DNA fragment was confirmed.
The correct sequences of these PCR-amplified products were confirmed by DNA-sequencing (data not shown).
Correct sequences of all plasmid inserts were confirmed by dRhodamine Terminator Cycle Sequencing Perkin-Elmer Life Sciencess, Boston, MA).
Other domains that might be characterised by generally preservative transmission are domains in which a final result is reached through a sequence of actions, and sequences of actions that are even slightly different to the correct one produce an unusable result (Acerbi et al. 2011).
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