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The correct mutation was confirmed by PCR.
Plasmids recovered from this procedure were sequenced to confirm that the correct mutation had been introduced.
The correct mutation status was identified when an Rm/Rwt ratio cut-off >0.7 was used; however, in 68 assays, a cross-reactivity signal was observed.
The correct mutation was verified by sequencing.
The presence of the correct mutation was confirmed by sequencing (The Sequencing Service, University of Dundee).
Introduction of the correct mutation and exclusion of other mutations were verified by Sanger sequencing of the whole coding region.
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Finally, this recent story, again by Ian Sample, is about a fascinating scientific advance that has allowed researchers to edit the genome of living animals (mice in this case) to correct mutations that cause an inherited blood disorder.
In laboratory studies, scientists have used genome editing – known technically as Crispr-Cas9 – to correct mutations that cause metabolic disorders, to create cells that attack tumours and to make others that are resistant to HIV infection.
Researchers in the US used a powerful new gene-editing procedure called CRISPR to correct mutations in the dystrophin gene in mice that were destined to develop the disease.
Genome engineering is an emerging strategy to treat monogenic diseases that relies on the use of engineered nucleases to correct mutations at the nucleotide level.
Using CRISPR to insert this same system into zygotes, Jaenisch's team reported making conditional mice with relatively "high efficiency"—about 16% of the zygotes led to mouse pups with the correct mutations.
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