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In particular, cloning efficiencies were represented as "the number of clones with the confirmed correct length of insert DNA by colony-PCR/number of colonies subjected to colony-PCR".
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Additional criteria were also used relative to the predicted length of insert based on human cDNA length, in order to avoid clones of relatively short insert length.
Colonies containing insert of correct length were cultured and the corresponding plasmids were purified by Qiagen spin miniprep kit (Qiagen) and sequenced.
Cloning efficiencies for the insert DNA were given as the ratio of colonies with an insert of the confirmed correct length as estimated by colony-PCR.
A stock with isolation number 6.1 was selected for correct orientation of insert pBSattBattPLoxFRTy.
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Purified plasmids were amplified using M13 primers to test for inserts with correct length.
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