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Selecting for integrants on YPD NAT plates resulted in strain KGY54, and correct integration of the TEV cleavage sites was confirmed by sequencing.
The correct integration in the APJ1 locus was verified by PCR.
The Appropriate Placement concept (also referred to as Correct Integration) is fundamental in Pinch Analysis.
The accurate modeling which includes the correct integration of the equations of motion is essential.
The correct integration of the expression vector was verified by PCR analysis and gel electrophoresis (Fig. 3b).
Correct integration at the ywpE locus was confirmed by PCR using ON54 and ON55, resulting in strain NDH31.
The correct integration of the fragment disrupting the coding sequence was further tested by PCR and sequencing.
Nevertheless, the correct integration of such parameters and their exposure as a clinical guide are still unresolved issues.
Correct integration was confirmed genetically.
Correct integration was confirmed by unambiguous PCR analysis.
Additionally, a fragment indicative of the correct integration event could also be detected (Fig. 3A).
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