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These plasmids were again digested by the suitable restriction enzyme followed by gel extraction and purification of the insert to confirm the correct insertion.
A miniature video camera on the laryngoscope's tip relays a picture to the screen on the device to help its correct insertion.
Correct insertion in the YLR446W locus was verified by PCR.
Plasmids with correct insertion and without mutations were selected.
Southern blot confirmed that in all three clones, both alleles of AAVS1 contained the correct insertion (Fig. 4A and 4B).
A prefabricated occlusal replica (Fig. 9) allowed the most correct insertion of two zygomatic implants (40 and 42.5 mm, respectively).
Verification of the correct insertion (APJ1 locus) was done by sequencing using primers Ver.ins1-F/Ver.ins1-R and Ver.ins2-F/Ver.ins2-R.
To confirm the correct insertion of the OX40L gene into the eYFP vector, polymerase chain reaction (PCR) was performed on the purified products.
Finally, the clone was sequenced to confirm correct insertion.
After plasmid minipreps (Qiagen), restriction digests and PCR were used to verify correct insertion.
The correct insertion of the selection markers was confirmed by PCR.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com