Sentence examples for correct incorporation from inspiring English sources

The subsequent insertion of the puromycin selection cassette, plasmid linearization, transfection and selection of DT40 lines that had incorporated the insert, and PCR confirmation of the correct incorporation, were all as described in Richardson et al. [ 18].

Immunofluorescence analysis revealed correct incorporation of WT and missense, but not of truncated cMyBP-C in the sarcomere.

Repaired cMyBP-C protein was detected by immunoprecipitation in cells and in vivo and exhibited correct incorporation into the sarcomere in cardiac myocytes.

As natural populations are invariably exposed to multiple stressors and genetic variability may change accordingly, it is essential to improve our knowledge on the effects of such scenarios in order to allow a correct incorporation in ecological risk assessment methodologies.

The correct incorporation of the GFP tag at the 3' end of the targeted kinesin coding sequence was checked by PCR (not shown) and immunoblotting of cell lysates with monoclonal antibodies directed against GFP (Fig. 2B).

The faster migrating 18-mer corresponds to the correct incorporation of A opposite T and the slower one corresponds to the misincorporation of G, consistent with the preferential incorporation of dGTP opposite T for this template/primer substrate by Pol ι [4].

We corrected incorporation values to the amount of DNA present in each culture to provide an index of DNA synthesis per cell (Winick and Noble 1965), and we recorded the total DNA content.

The additional use of a 0.6 kb PCR product from the neomycin resistance gene as a probe yielded a hybridisation signal of 6.8 kb, thereby confirming the corrected incorporation of the PGK-Neo cassette in transfected ES cells.

Fluorescence emission was monitored using a 360 nm high pass filter (Corion, MA) on a logarithmic time scale for 1 s for correct dNTP incorporation and up to 50 s for mismatched dNTP incorporation (depending on exact conditions).

For example, only half of the modified protein sfGFP212pPaF contained the correct pPaF incorporation, whereas the other half of the protein was mis-incorporation of E Supporting Information Figuree S2b).

For the unmodified sequence, reactions were performed in a shorter time period of 1 min for correct nucleotide incorporation and for up to 45 min in the case of incorrect nucleotide incorporation.