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Another study used qPCR only to address discrepancies in 11 genes identified as differentially expressed [ 2], observing that sequencing made 4 of 5 correct calls of differentially expressed genes while arrays correctly identified 2 of 6 differentially expressed genes.
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Subjects attempt to guess correctly the symbols of cards hidden from their view under controlled conditions; a better-than-chance percentage of correct calls on a statistically significant number of trials is considered to be evidence of ESP.
With two of the six false calls resolved the assay delivered 188 correct calls out of 192 specimens tested, or an accuracy of 97.9%.
It consequently provides for high accuracy identification of inherited (98% accuracy) but not de novo single nucleotide variants (17 correct calls out of 44 true de novo sites, with 3884 called positions).
But on election night he tried to bully Fox News into retracting its correct call of Ohio — and hence, in effect, the election — for Mr. Obama.
The correct calling of poor performing SNPs may prove to be key in future linkage studies performed on the 10K GeneChip.
To ensure correct calling of heterozygote genotype we used an additional PCR reaction with primers 5′-AGGCTGCATTGAGGC-3′ (Common L primer) and 5′-TCCGTAACAAGATCTGATGCCCTC-3′ (MinB2R, in B2 sequence) to assay the presence or absence of Comt1 B2i.
Under this assumption, if the true base is A, an observation of M could result either from a correct call of A (probability 1 − ε) or an erroneous call of C (probability ε/3) and therefore has probability 1 − 2 ε/3, and similarly if the true base is C.
But for a handful of botched calls there are countless correct calls on close plays.
So iconic that McEnroe named his own autobiography after the outburst, technology could've made the correct call on one of sport's most memorable moments right there and then.
However, because chromosome confidences computed by PS are indicative of the probability of correct calls (Table III), confidences can be used as a proxy to assess the quality of the measurements.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com