Wheat, barley, oats, peanuts, red apples, and corn encourage yeast growth in the body because of their high glucose content, which is food for yeast.
Four significant variables (corn starch, yeast extract, corn steep liquor, and inoculum size) were selected for the optimization studies.
Standard Drosophila food contains high amounts of carbohydrates (sugar and corn) and yeast, the latter providing mostly amino acids and fatty acids, but also compounds for which flies are auxotrophic.
Media containing soybean, casein, gelatin, corn, and yeast are commonly used to produce protease.
Fly cultures were kept at 25°C with a 12-h light-dark cycle on corn meal, yeast, sucrose, and agar food.
For each line, five males and ten females were maintained at 20°C and ~75% humidity on standard Drosophila medium (containing molasses, corn meal, yeast, agar, and antimicrobial agents).
These flies were kindly provided by Dr. M.T. Kimura and maintained on standard fly media (molasses, corn meal, yeast extract, and agar) in a 25° incubator.
Flies 3 dpe were grown in corn meal yeast fly food supplemented with 200 μl of 6-mg/ml BrdU in PBS for 2 days before infecting with E. coli.
Fly stocks were maintained at room temperature on corn meal-yeast-agar medium obtained from the University of Arizona fly food facility.
Experimental cultures were reared at 25°C and 60 80% relative humidity on corn flour-yeast-agar medium as previously described (Kraft et al., 2006).
