Cells were propagated and maintained in 75-cm2 flasks (Corning CellBind Surface, Corning, NY).
A suggestive, dangerous subtext belies its down-home, corn pone surface.
Fig. 7 a Variation of water contact angle and oil contact angle of superhydrophobic/superoleophilic corn straw surfaces in aqueous solutions with different pH values.
For the intracellular calcium measurement assay, cells were seeded in 24-well microplates (Corning CellBind Surface) at a density of 10 cells/well.
Corning cellBIND surface 96-well plates as well as culture medium, poly- d-lysine (Mw>300 kg/mol), ionomycin, MTBSTFA (N-tertbutyl-dimethylsilyl N-methyltrifluoroacetamide) containing 1% tertbutyl-dimethylchlorosilane were from Sigma Aldrich.
For Nampt/PBEF/visfatin expression analysis, 3 × 10 cells/well were seeded on plastic, flat bottom, 6-well plates (Corning CellBIND surface) and cultured until 80% confluence at 37°C in a humidified atmosphere containing 5% CO2.
At about 80% of confluency the MSCs were harvested by accutase treatment (PAA Laboratories GmbH, Pasching, Austria) and plated at a density of 3000 cells/cm2 in 25 cellculturelture flasks (Corning, CellBind Surface, Germany) and in 6-well plates (Sarstedt, Germany), respectively.
Dissolved oxygen and pH values in the cell culture supernatant were recorded online in 25 cellculturelture flasks (Corning, CellBind Surface, Germany) every 10-20 minutes by using a SFR-Shake Flask Reader (Presens GmbH, Regensburg, Germany) with optical sensors, integrated and precalibrated by Presens GmbH.
23 Cells were plated in 24-well plate with Ultra-Low Attachment surface (Corning Inc., Corning, NY, USA) at a density of 2.5 × 10 cells per well and cultured for 21 days in RPMI1640 with 10% FBS and 0.3% agarose.
Because air was captured and trapped, while falling onto corn straw fiber surface, by the abundant cavities and interspaces among SiO2 particles on the fiber surface, a water droplet could contact the trapped air to manifest a non-wetting phenomenon.
