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Amongst organic sources, yeast extract (YE) was favorable for EPS and cordycepin production, while corn steep powder (CSP) was favorable for adenosine production.
The absence of (NH4 2SO4, the use of corn steep powder and yeast extract as composite organic nitrogen source were more suitable for cell growth and l-phenylalanine production.
The medium applied in the transformation experiments was prepared using distilled water and composed of following components: glucose 20 g/L, corn powder 15 g/L, wheat bran 5 g/L, KH2PO4 2 g/L, MgSO47H2O 3 g/L, yeast extract 2 g/L and corn steep powder 5 g/L.
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For nitrogen source screening, peptone, spray-dried corn steep liquor powder (CSLP), malt extract powder, urea, ammonium sulfate, and sodium nitrate were tested.
XT15 preferred organic nitrogen sources including corn steep liquor powder, a cheap by-product from corn wet-milling.
At 55°C, 7.7 g/L of acetoin and 14.5 g/L of 2,3-butanediol could be obtained using corn steep liquor powder as a nitrogen source.
Corn steep liquor powder (CSLP), an inexpensive organic nitrogen source available on a large scale, was used to replace the expensive organic nutrient in the selected medium.
Our data showed that the erythromycin production was little affected by the substitution of corn steep liquor with yeast powder.
The total nitrogen contents in the two media were the same because the substitution of corn steep liquor with yeast powder was based on the equal nitrogen principle.
These phenomena could result from the difference in terms of the ingredients between yeast powder and corn steep liquor.
By comparing the process parameters between the medium with yeast powder and the medium with corn steep liquor, the significant differences were observed that the fermentation with yeast powder medium had a much lower concentration of soluble phosphate and a much higher amino nitrogen level during the early fermentation stage as compared with that using corn steep liquor medium (Figure 1A, B).
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