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We are part of a corn culture, too.
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Flies are maintained in laboratory by mass crosses and cultivated in corn flour culture medium in a constant temperature chamber (20 °C).
The partners, who in conversation don't finish each other's sentences so much as escalate one another's tangents, also shared a fascination with what they perceive as the unsettling underbelly of corn-pone culture.
Biomass dry weight of corn straw cultures was indirectly measured as previously described [ 46].
Transfected cells were plated to confluence on Primaria 24-well dishes (Corning) in culture media.
Cells were grown in 35-mm-diameter Corning tissue culture plates to the same confluency as the SL/DT assay.
Cells were seeded in a T75 flask (Corning) containing culture media and maintained at 37 °C with 5% CO2.
An 80 90% knockdown of UCP2 protein was accomplished through siRNA transfection of INS-1E cells grown on XF24 (Seahorse Bioscience) or 96-well (Costar 3595; Corning) tissue culture plates.
Cells were plated onto 25 cm Corning cell culture flasks or polystyrene six-well flat-bottom microtiter plates and were incubated at 37°C, 5% CO2/95% air, and 95% humidity for 4 hr to allow the macrophages to adhere to the surfaces.
Patches were transferred into six-well ultra-low adherence plates (Corning) in hES culture medium and cultured for 3 days at 37°C.
Cells were maintained in 100 mm × 20 mm Corning tissue-culture dishes (Plainfield, NJ) at 37°C and 5% CO2 in humidified chambers.
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