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To identify SG specific modulators, SG cores were isolated according to the well-established procedures (Jain et al., 2016) from the TIA-1 overexpressing HeLa cells.
Mononucleosome cores were isolated from MNase-digested chromatin as previously described [ 4, 6].
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Brains were then removed from skulls, and tissues in the PL and NAcc core were isolated from other parts of the brains structures.
And while a reactor core is isolated by thick layers of steel and concrete, a spent-fuel pool is largely unshielded.
In 2008, -aspergillide C, a structurally novel marine metabolite bearing a 14-membered macrolactone around a 2,6-dihydropyran core, was isolated from a marine-derived fungus, Aspergillus ostianus.
However, in the penumbra region, processes of Iba-1+ cells became thick and short (arrowheads in Figure 2d), and cells near the core appeared to be arranged so that the core was isolated (white arrows in Figure 2c, d).
RNA from one third of the carotid plaque core was isolated using Trizol (BRL-Life Technologies), FastPrep (MP Biomedicals), and RNeasy Mini kit (Qiagen) with a DNase 1 treatment step (Qiagen).
Wild-type and mutant E. coli RNAP core enzymes were isolated according to Kashlev et al (1996).
For teratomas derived from individual C4955, core sections were isolated from FFPE embedded teratomas tissue using a 3 mm dermal punch tool; DNA was extracted from core samples using a QIAamp DNA FFPE Tissue Kit (Qiagen).
Hepatocytes infected with HBV and expressing HBV core protein were isolated from CHB patients, immunoprecipitated with HBc antibody, and investigated to determine the genome-wide profile of bound host gene promoters.
In order to determine the involvement of DDX3X in the HCV viral life cycle, protein fusions to HCV core protein regions were isolated that interact with DDX3X both in vitro and in vivo.
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