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The cores were fixed in 4% glutaraldehyde and the species composition of the microalgal community was assessed within 10 subsamples per sample by light microscopy.
Cores were fixed with 0.2 µm pre-filtered glutaraldehyde solution (1% final concentration) and bacteria were stained with Syto13 (Molecular Probes, 1∶2000 v: v, 1.2 µmol l−1 final concentration) for 15 min in the dark.
The cores were fixed in 10% formaldehyde-saline and processed according to routine histological techniques, until paraffin embedding.
The lids were screwed tightly onto the vials to limit evaporation, and the biopsy cores were fixed in the aqueous alcohol solution at room temperature, without shaking, for 24 hr.
The same experimental procedure with virus vehicle was applied for the Group 2. At the end of the 6-hour infection, part of the infected and control cores were fixed in RNAlater (Ambion, USA) for assessment of the quantitative expression of genes by the qRT-PCR method.
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The absorption values of the CdTe core were fixed at 0.6.
Minimum diameter of the central core is fixed and the parameters to be explored are the number of fins, their thickness, length (radial dimension) and height.
Note that the conductivity of the core is fixed to 105 S/m.
This would remove the damage on the core surface and would not affect the core permeability of the non-damaged area since the core was fixed inside the round tube using two O-rings and a heavy duty glue.
In conclusion, according to the Preisach Theory, CT core model can be established accurately by measuring the descending branch of limiting hysteresis loop and positive saturation magnetization curve, when structure of CT's core is fixed and the outside temperature does not change obviously.
The molar ratio of [Cd2+]/[Se2-] was set as 1 1, the absorption value of the CdTe core was fixed at 0.6, and Figure 3c shows the PL intensities of the CdTe/CdSe QDs with different ratios of the MPA stabilizer to the concentration of Cd2+.
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