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Protein expression of each core was assessed using the weighted histoscore method (Kirkegaard et al, 2006).
Long-term post-ictal recovery of units in the ictal core was assessed using the 2 h immediately following seizure termination, as a single epoch.
Expression of FGFR1 in each core was assessed using the weighted histoscore method (Edwards et al, 2003), grading staining intensity as negative (0), weak (1), moderate (2) and strong (3), before multiplying by the percentage of tumour cells within each category.
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The depth of coverage for a specific core domain was assessed using the Miller's Triangle as a reference [ 8].
The feasibility of the PedsQL™ 4.0 Generic Core Scales was assessed using the percentage of missing values and rate of completion of scales [ 11, 12, 15].
Core body temperature was assessed using a rectal temperature probe.
The presence of highly conserved core eukaryotic genes was assessed using CEGMA 2.5 [ 29, 63].
Core body temperature was assessed using a portable telemetry system (CorTemp™ system, HQ Inc, Palmetto, USA), which has been demonstrated to be safe and reliable (Byrne and Lim 2007; Gant et al. 2006).
The protein expression of each core (three per tumour specimen) was assessed using the weighted histoscore method (H score method).
The SFK member expression of each core (three per tumour specimen) was assessed using the weighted histoscore method (H-score method (Kirkegaard et al, 2006)).
The activity of the core TGF-β signaling pathway was assessed using TGF-β1 binding assays, phospho-specific Smad antibodies, immunofluorescent staining of Smad and Smad DNA binding assays.
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