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A core set of lines from the population of 632 lines studied here was created that captures the maximum diversity of the SNP haplotypes.
A core set of lines was chosen to see how many lines were necessary to capture 90% of the allelic diversity present in the entire set.
Consequently, for association mapping in breeding populations, substantial added value can be generated by complementing routine genotyping performed on an economic marker platform with high density genotyping of a core set of lines for subsequent data imputation.
To identify candidate loci linked to genomic regions that might have experienced divergent selection, we used the 92 fiber flax accessions present in the core collection (Additional file 6: Table S3) The "line selection" module in PowerMarker v.3.25 [ 63] allows the selection of a core set of lines from a large germplasm collection that maximizes the genetic diversity.
Both without and with controlled vernalization, BdTR3c was the tallest in the core set of lines, indicating that this height difference is at least partially under genetic regulation, rather than being simply the secondary effect of flowering time or environmental differences.
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Markers that were heterozygous in the F1 and segregating in an F2 subset of 9 lines were then tested on a core set of 93 F2 lines.
In a separate assay, copulation duration was assayed and compared with MMP assays for 35 DGRP lines chosen from the "core set" of 40 DGRP lines with the first broadly available sequence data.
After identification of polymorphisms (SNPs and indels) within the core set of 10 inbred lines, a total of 21 candidate genes were selected to be further genotyped in the AMP.
Using our approach, we first selected the global core set of seven sequences (black lines), then the local P-core set of 10 sequences (blue lines), and then eight sequences (red lines) with the local S-core set.
A core set of 168 B. rapa lines has been identified and used in a genome wide association study using predominately AFLP markers [ 45].
In total, the core set of RIL populations included 1,031 lines.
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