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Two cylindrical specimens were produced from each liner using a 9 mm diameter core punch.
At least two tumor cores were extracted from areas containing representative invasive breast carcinoma using a 1 mm core punch.
Tissue microarrays were constructed using a semiautomatic array machine (ALPHELYS MiniCore 3, Plaisir, France) using a 1-mm core punch, annealed at 40 °C.
Some studies have enriched tissue samples for specific cellular content (cancer cells vs stroma) using laser capture microdissetion [ 57 60], a tissue core punch extractor [ 61] or tissue macrodissection [ 62] before miRNA detection with high-throughput and sensitive methods.
FFPE tissue specimens were analysed histologically to identify tumour-rich regions, and samples (n=3) were taken using a 1-mm diameter core punch with the MTA-01 personal tissue arrayer (Beecher Instruments, Sun Prairie, WI, USA).
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Building on its core punch-card technology, Watson put the company on the fast track by focusing on landing large-scale custom projects with big business clients.
Core punches, 1 mm in diameter, were drilled from representative areas contained within each FFPE tumor blocks.
A central core and a peripheral 1 mm TMA core punched from the area previously identified as including invasive tumour were transferred to TMAs from each of the 54 paraffin blocks.
Since the normal tissue samples contain a heterogeneous mixture of stromal and epithelial tissue, normal samples were assayed as FFPE tissue scrolls (10 um thickness) rather than core punches.
For enlarging cored, punched, or drilled holes, core drills are particularly suited.
Ten selected blocks of the same specimen were sampled for RNA extraction using four 1 mm cores punched out from tissue blocks with TMA Master.
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