Some of these identified motifs were matched to known motifs in the plant transcription factor binding sites database, JASPAR CORE plants [ 39, 40].
The first one matched to a known zinc finger motif and the last two were identified as leucine zippers in the database of plant transcription factor binding motifs, JASPAR CORE plants [ 39, 40].
Then, we concatenated and evaluated rbcL and matK together, as this is the core plant DNA barcode proposed by the CBOL Plant Working Group [ 5].
trnH- psbA was rejected as a core plant barcode because of difficulties in amplification and sequencing [ 5], and because inverted repeats may also be prevalent [ 45].
We measured the efficacy of the core plant DNA barcode regions (matK and rbcLa) (CBOL 2009) to identify African Psoraleoid legumes using the two approaches: 'barcode gap' and discriminatory potential (Meyer and Paulay 2005).
Control of the core plant cell cycle genes at the transcriptional level has been associated with regulation of the cell cycle in synchronised Arabidopsis and tobacco BY2 cell cultures [ 30, 36- 38].
Specifically, the Plant Working Group of the Consortium for Barcodes of Life has proposed the combined use of short segments of the chloroplast genes matK and rbcL as a "core" plant barcode [ 5].
The Populus genome sequence and the findings reported here provide new opportunities to facilitate postulation and exploration of hypotheses linking auxin response regulatory genes to conserved core plant processes, as well as perennial plant features such as wood development, long-distance nutrient and water movement, seasonal dynamics, and disease resistance.
The present study examines the role that DNA barcoding can play in aiding floristic evaluations in the arctic by testing the effectiveness of the core plant barcode regions (rbcL, matK) and a supplemental ribosomal DNA (ITS2) marker for a well-studied flora near Churchill, Manitoba.
To remove a stem from the crown, use a back and forth pulling motion and not a side-wise motion (hold the core plant in one hand, and grab the stem in the other, rotate the stem-holding back and forth), work the stem back and forth until the rhizome comes loose or breaks from the crown.
As an example of this phylogenetic variability of observed relative frequencies of RSs, we observed 45.8 recognition sequences per megabase (RS/Mb) ± 24.6 (mean ± standard deviation [SD]) for NgoMIV in core eudicot plants, compared with 277.4 ± 131.3 RS/Mb in commelinid plants (monocots).
