These were attributed to enhanced fibrillation of the liquid crystalline polymer (with fibril formation observed along the core regions of injection moulded parts also) when injection moulded in the presence of the terpolymer and improved interfacial adhesion characteristics between the components of the in situ composite.
For nanoindentation creep on the skin and core regions of injection moulded samples, whilst organoclay improves the creep resistance of PA 6, the enhancement is due to the decrease of the initial compliance at zero time but the time-dependent creep is actually increased.
In the core of the injection site, there was extensive labeling of neural and glial structures with confluent labeling that made distinguishing individual cellular profiles difficult (Figure 1A).
As in the wt animals, in hCAR animals we observed extensive transduction in the region of the SNc; at the core of the injection site the labeling was essentially confluent, with staining of many cells with astrocytic morphology (Figure 1B).
Direct quantitation of the number of neurons in the SNc infected by Ad5 proved impossible due to the extent of GFP expression in surrounding non-neuronal cells, particularly in the core of the injection location where expression was most robust (Figure 1).
The air injection needle was placed at a depth of 80% or 88% of total corneal thickness; however, this value represents the centre core of the injection needle.
At late stages, MSK-1, DARPP-32 and NeuN staining was preserved in rare neurons located in the core of the injection.
Finally, DARPP-32 loss over Exp-Htt was reduced at this stage, indicating that MSK-1 overexpression prevented the extension of the lesion produced by very high expression of Exp-Htt in the core of the injection.
At 10 weeks, PGC-1α expression was strongly reduced in the core of the injection on the Exp-Htt side compared with the Htt side (Exp-Htt, 2.29 versus Htt, 139.43; *** P< 0.0001) (Fig. 5D).
On the co-infected side (Fig. 2A, right panel), this down-regulation was evident in the core of the injection, while the surrounding area showed MSK-1 expression and DARPP-32, along with Exp-Htt expression (Fig. 2B, right panel).
On the LV-Exp-Htt/MSK-1-coinfected side, we dissected out the core of the injection and the surrounding area and found a slight but significant increase in PGC-1α expression in the core of the injection (Exp-Htt, 2.29 versus Exp-Htt + MSK-1, 36.14; *** P< 0.0001) and a strong increase in the surrounding area [Exp-Htt, 18.29 versus Exp-Htt + MSK-1, 299.57; *** P< 0.0001 (Fig. 5D)].
