Music: As befits the space, a hard-core mix of hip-hop and house at its loudest — overlaid with Hines' high-volume patter.
In preselective PCR, 2 μL of diluted restriction/ligation product was added to 7.5 μL of AFLP core mix (Applied Biosystems), 0.25 μL of the EcoRI core sequence (5′-GAC TGC GTA CCA ATTC-3′), and 0.25 μL of the MseI core sequence (5′-GAT GAG TCC TGA GTAA-3′).
Primer testing was performed using 125 ng of a commercial pool of human genomic DNA (Roche Applied Science, Indianapolis, IN) in a PCR core mix containing 200 nM of each forward and reverse primers, 1X AccuPrime™ Buffer II (Invitrogen, Carlsbad, CA) and two units of AccuPrime™ Taq DNA Polymerase High Fidelity (Invitrogen).
"You mix that young with the older guys and you get that great core, great mix of players.
For the randomized 12 N core a mix of dATP, dTTP, dCTP and dGTP in a ratio 1 1 1 1 was used during synthesis.
Schelling described the group of leading thinkers from around the School as "distinguished misfits". The School's core was a mix of political scientists, statisticians, economists, and decision theorists that included himself, Richard Neustadt, Philip Heymann, Howard Raiffa, Fred Mosteller, and Francis Bator.
A novel fission fusion hybrid reactor was proposed, comprising saturating of a fissile actinide core with a mix of deuterium tritium hydrogen isotopes (U D T target), similarly to Hyperion design.
If you look back to the early 1900s, city centers were delivering what people want today in the urban core--a mix of residential, shopping, entertainment, meaningful public spaces.
Parallel to viability assay, infection in Group 3 had occurred where infected cores exhibited a mix of live and dead morphology following 48 hours in culture.
This study was conducted to investigate the permeability reduction caused by deposition of barium sulfate in sandstone cores from mixing of injected seawater and formation water that contained high concentration of barium ion at various temperatures (50 80 °C) and differential pressures (100 200 psig).
This study was conducted to investigate the permeability reduction caused by deposition of barium sulfate in Malaysian sandstone and Berea cores from mixing of injected Malaysian seawaters (Angsi and Barton) and formation water that contained high concentration of barium ion at various temperatures (50 95 °C) and differential pressures (75 200 psig).
