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They use a core mix -- celosia, nigella, straw flowers, larkspur and gomphrene -- and each year try out something new.
Preamplification was performed in 15 µl 1×AFLP Amplification Core Mix Module (Applied Biosystems, Foster City, CA, USA) supplied with 0.12 µM Eco+A primer (5'-GACTGCGTACCAATTCA-3'), 0.92 µM Mse+C primer (5'-GATGAGTCCTGAGTAAC-3'), and 2 µl undiluted restriction-ligation product as template.
In selective PCR, 1.5 μL of diluted preselective PCR products was mixed with 8.5 selective PCR mix containing 0.5 μL EcoRI-AC (labeled with FAM [6-carboxy fluorescein]), 0.5 μL MseI-A, and 7.5 μL AFLP core mix (Applied Biosystems).
Primer testing was performed using 125 ng of a commercial pool of human genomic DNA (Roche Applied Science, Indianapolis, IN) in a PCR core mix containing 200 nM of each forward and reverse primers, 1X AccuPrime™ Buffer II (Invitrogen, Carlsbad, CA) and two units of AccuPrime™ Taq DNA Polymerase High Fidelity (Invitrogen).
In preselective PCR, 2 μL of diluted restriction/ligation product was added to 7.5 μL of AFLP core mix (Applied Biosystems), 0.25 μL of the EcoRI core sequence (5′-GAC TGC GTA CCA ATTC-3′), and 0.25 μL of the MseI core sequence (5′-GAT GAG TCC TGA GTAA-3′).
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Music: As befits the space, a hard-core mix of hip-hop and house at its loudest — overlaid with Hines' high-volume patter.
Multi-core mixed-criticality systems are complex solutions that provide benefits regarding lower power consumption, size, weight and cost and better performance and scalability, compared with single-core architectures.
The incident was apparently set off when one or more of the 3,200 pipes inside the No. 24 generator leaked, allowing perhaps 5,000 gallons of radioactive water heated by the nuclear core to mix with clean water used to generate steam and run turbines, Mr. Quinn said.
For the randomized 12 N core a mix of dATP, dTTP, dCTP and dGTP in a ratio 1 1 1 1 was used during synthesis.
The city has recently voted to create a new central core that mixes sustainable housing and mixed-use development.
Expect to find an additional 20 or so on the "core" menu, mixing popular varietals from small producers and odd grapes, plus dozens of by-the-bottle choices on a menu dubbed "The Great Big Bottle Book". The daily Aperitif Hour, from 5 to 7 p.m., brings a free glass of aperitif, such as vermouth, for everyone.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com