Sentence examples for core mediators of from inspiring English sources
In silico substrate modeling in this putative binding pocket suggests a mechanistic sequence relying on H291, K295, and Zn2 + as core mediators of catalytic turnover.
Ideally these targets should be core mediators of important processes and not downstream components of the response [21].
IL-18 is a core mediator of angiogenesis and inflammation in rheumatoid arthritis pannus formation [ 28].
MED26 is not the only subunit of the core mediator to depend on IFN-I for recruitment to the Nos2 and Il6 promoters.
Our data suggest that the functional cooperation between two major antimicrobial pathways is based on promoter priming by NF-κB and the engagement of the core mediator for Pol II binding by ISGF3.
To determine whether this extends to subunits of the core mediator, binding of the tail subunit MED5/24 was examined.
After unambiguously fitting the crystal structure of the Head module complexed with RNAPII CTD (PDBID: 4GWQ) into the 3D map of the core Mediator, the orientation of the RNAPII CTD binding to the core Mediator could be directly deduced.
A detailed comparison of the structures of the core Mediator and its subassemblies, combined with nanogold labeling of multiple Mediator subunits of different modules, suggests that the Head + Middle modules form the upper portion of the structure, while the Tail module forms the dense domain at the base.
MED1 is part of the core mediator module while MED12 is part of the mediator kinase module [ 74, 75].
The gold clusters clearly pinpointed the C-termini of the Med17 and Med22 subunits at roughly the same position on the upper-right portion of the core Mediator, which directly validates the assignment of the Head module.
The limitations of the previous segmentations were that they were based solely on comparisons of low-resolution EM structures of the core Mediator associated with RNAPII and in free forms, which were very ambiguous; furthermore, none of the individual Mediator subunits was localized in the EM structure.
