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The aim of this study was to determine the reproducibility of cardiac safety assessments across two academic echocardiography core laboratories (ECLs) at the University of Pennsylvania and the Duke Clinical Research Institute.
This national, multicenter study was designed to prospectively evaluate stable patients able to undergo CTCA followed by conventional CA. Data from CTCA and CA were analyzed in a blinded fashion at central core laboratories.
The core laboratories in the research complex, at the Anschutz Medical Campus, include mass spectrometry, X-ray crystallography, electron microscopy, a 900 mega-hertz nuclear magnetic resonance (NMR) spectrometer, DNA array and peptide protein chemistry.
rtRT-PCR reactions were performed using the SuperScript III Platinum One-Step qRT-PCR kit (Invitrogen) under the same conditions in POC and core laboratories.
We thank the staff in the Medical College of Georgia cell imaging and histology core laboratories for assistance in the use of their facilities.
With a common set of specimens and centrally prepared stimuli and controls, the findings from this evaluation have justified the continued use of the two different SOPs within the core laboratories of the CTC-VIMC.
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DPT-1, TrialNet, and TEDDY studies used single-core laboratories that processed HbA1c using the National Glycohemoglobin Standardization Program (NGSP -certified method.
Frozen serum samples will be analysed at the two core-laboratories at the Department of Endocrinology and Internal Medicine, Aarhus University Hospital, and the Department of Endocrinology and Internal Medicine, Herlev University Hospital, Copenhagen.
We thank the Genomic Proteomics Core Laboratories of the University of Pittsburgh (supported by the Clinical and Translational Science Institute) and the Flow cytometry Core, Children's Hospital of Pittsburgh for technical expertise.
The samples were sent out for sequencing to Sequencing and Genotyping Core Facility, Genomics and Proteomics Core Laboratories, University of Pittsburgh.
After the cores were cleaned with tobuence for several days, the displacement experiment was carried out using an AFS-300 displacement system developed by Core Laboratories.
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