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Buccal cell and CD4+ lymphocyte DNA extractions were performed using Puregene buccal cell core kits (Qiagen Sciences, Germantown, Maryland, United States) according to the manufacturer's instructions, except all centrifugations were conducted at 4°C instead of room temperature.
Buccal cell DNA extractions were performed using Puregene Buccal Cell Core Kits (Qiagen) according to the manufacturer's instructions, except all centrifugations were conducted at 4°C instead of room temperature.
RNA extraction was performed using the Freedom EVO® 150 (Tecan, Grödig, Austria) automated platform and the Nucleospin® 96 Virus and the Nucleospin® Virus Core kits (Macherey-Nagel, GenXpress, Wiener Neudorf, Austria) for serum and tissue samples, respectively, following the instructions of the manufacturer.
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Real-time PCR was performed using a real-time PCR Taq core kit (Takara, Dalian, China).
RT-PCR was performed using PCR Taq core kit (Takara Bio Inc., Dalian, China).
Printoo kits start at $45 for a core kit (which consists of three components) or $95 for a basic pack (seven components).
Reverse transcription was completed using GeneAMP RNA PCR Core kit (Applied Biosystems) with 0.75 µg of RNA.
QPCR was performed using a qPCR Core kit for SYBR Green I (Eurogentec, The Netherlands).
Multiplex PCRs were performed using a Taq PCR core kit (Qiagen, UK).
Quantitative real-time RT-PCR was performed using one-step qPCR core kit (Eurogentec).
Generation of T-REx-c-MIR cells was performed using the Flp-In ™ T-REx ™ Core Kit (Invitrogen).
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