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Additional ADCC experiments were performed with the ADCC Reporter Bioassay, Core Kit (Promega), according to manufacture instructions.
Real-time PCR was performed using a real-time PCR Taq core kit (Takara, Dalian, China).
RT-PCR was performed using PCR Taq core kit (Takara Bio Inc., Dalian, China).
Printoo kits start at $45 for a core kit (which consists of three components) or $95 for a basic pack (seven components).
QPCR was performed using a qPCR Core kit for SYBR Green I (Eurogentec, The Netherlands).
Multiplex PCRs were performed using a Taq PCR core kit (Qiagen, UK).
Quantitative real-time RT-PCR was performed using one-step qPCR core kit (Eurogentec).
Reverse transcription was completed using GeneAMP RNA PCR Core kit (Applied Biosystems) with 0.75 µg of RNA.
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and the qPCR products detected using SYBR Green Coree kit, Eurogentec) incorporating a melt curve analysis for each run.
Briefly, double-stranded cDNA was synthesised from total RNA and was labelled with the RNA Fluorescence Labeling Core kit (Takara Bio).
cDNA was generated in a total volume of 20 μl with 50 ng total RNA using GeneAmp® Gold RNA PCR Core kit (Applied Biosystems).
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