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All core gene clusters from 73 Salmonella genomes were used for generating a consensus tree.
Most (93%) of these core gene clusters contained a single representative from each strain.
The variable gene clusters are increasing and the core gene clusters are decreasing (Fig. 2e).
In order to determine the functional profile of core genes, the core gene clusters were aligned against UniProt [ 30].
The core gene clusters were compared in an all-against-all BLAST with protein sequences from UniProt based on the '50/50 rule' [ 30].
When all Salmonella genomes have been considered, there are 10,581 pan gene clusters and 2,882 core gene clusters (Additional file 2) in species enterica.
Similar(41)
The core gene cluster for cercosporin biosynthesis includes genes encoding a polyketide synthase, two O-methyltransferases, monooxygenase, an MFS transporter, three oxidoreductases and a Zn II 2Cys6 transcription regulator (CTB8).
Multiple alignments for each core gene cluster from all strains were performed using MUSCLE [ 40].
The resulting average identities within each core gene cluster is displayed in Figure 2B.
In Streptococcus thermophilus, a Gram positive species and therefore devoid of lipopolysaccharide, the exocellular polysaccharide biosynthesis cluster is composed of core gene cluster, represented by deoD- epsABCD, and followed by a variable region [ 40].
To test if this notion is broadly applicable to all pathogen-specific genes we compared clusters of pathogen-specific accessory genes to clusters of core genes (clusters comprised of exactly one of each lineage of Streptococcus).
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