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"Those original E.T.F.'s were broad, stock-based index-traded funds that were really efficient ways to gain core exposure to core markets".
After HCV core exposure (12 hr), HFµφ expressed elevated levels of CXCL10 and CXCL8 transcripts (Figure 3A and 3C, p<0.05).
However, HCV core exposure (12 hr) increased IL-6, TNF-α, CXCL10 and CXCL8 expression from 2 to 29 fold compared to Gal-exposed cells (Figure 6A, p<0.05).
Of interest, we also observed that HCV core exposure to microglia induced the transcript levels of indoleamine 2,3-dioxygenase (IDO) (data not shown), which has been linked to neuronal injury [68], [69].
Thus, availability on the web under an open license, machine readability and use of non-proprietary formats form the core exposure requirements for public big data resources.
Because some of the efficient design options for linking health outcomes to exposure metrics are outcome dependent, collecting basic (or core) exposure measures from all study subjects in a consistent manner with a sampling plan that provides coverage across life stages will be critical.
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6 hr after Gal or Gal-core exposure, HFNs were lysed in lysis buffer [20 mM Tris, 1% NP-40, 50 mM NaCl, Protease Inhibitor cocktail set III (1∶1000, Calbiochem, La Jolla, CA)].
HCV core protein exposure caused neuronal injury through suppression of neuronal autophagy in addition to neuroimmune activation.
Of interest, HCV core protein exposure did not activate the expression of interferon-alpha (IFNα) transcripts in both glial cell types (data not shown).
HCV core protein exposure was not toxic to astrocytes (Figure 4A) while the highest concentration of HCV core protein (100 nM) was cytotoxic to neurons, indicated by the loss of β-III-tubulin expression compared to controls (Figure 4B, p<0.05).
HCV core protein exposure induced expression of pro-inflammatory cytokines including interleukin-1β, interleukin-6 and tumor necrosis factor-α in microglia (p<0.05) but not in astrocytes while increased chemokine (e.g. CXCL10 and interleukin-8) expression was observed in both microglia and astrocytes (p<0.05).
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