Step II: The kernel code disassembly.
The design information of a product can have significant impact on addressing the various issues in product remanufacturing, from upstream design for remanufacturing, to downstream remanufacturing processes, including disassembly, core refurbishment and remanufacturing process planning, etc.
We use PtK2 cells grown on EM grids infected for short times, followed by rapid freezing to gain ultra-structural insights into the VV core upon its disassembly.
We took advantage of this to study the structure of VV further, focusing on structural details of the core during virion disassembly.
MLC-GFP was recruited around the dissociating actin core during the disassembly of podosomes.
Okuwaki, M., Kato, K., Shimahara, H., Tate, S. & Nagata, K. Assembly and disassembly of nucleosome core particles containing histone variants by human nucleosome assembly protein I. Mol Cell Biol 25, 10639 51, https://doi.org/10.1128/MCB.25.23.10639-10651.2005 (2005).
The study is conducted by ignoring the passive safety features incorporated in the design so that the accident scenario culminates in an energetic disassembly of the core with large energy release.
Our study shows that cell attachment induces several rearrangements of the viral core to form a distinct disassembly intermediate.
Following uncoating and disassembly of the core particle, the viral DNA is delivered to the nucleus and converted into covalently closed circular (ccc) DNA.
