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Last summer, AFP spent a half a million Koch dollars to elect anti-Common Core candidates to local office.
The total lengths of core candidates were 239.6 Mb (Mx23Hm) and 353.5 Mb (0431-1), whereas those of line-specific sequences were 273.4 Mb (Mx23Hm) and 358.6 Mb (0431-1) (Fig. 1 and Supplementary Table S4).
The numbers of rRNA-encoding genes identified in the core candidates (65 in Mx23Hm and 76 in 0431-1) were less than those in line-specific sequences (411 in Mx23Hm and 223 in 0431-1).
Considering that the purpose of this study is to survey as much genomic regions as possible to assign core candidates and line-specific sequences in the I. trifida genome, SOAPdenovo2 r223 was chosen for the following analyses.
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Core candidate motifs identified with probability scores >0.8 in human and at least one other species were chosen for further experimental analysis.
The total length of core candidate sequences in 0431-1 was longer than those in Mx23Hm.
Of the assembled sequences, 240 Mb (Mx23Hm) and 353 Mb (0431-1) were classified into core candidate sequences.
The core candidate proteins are sorted by the numbers of their own always active neighbors in nonincreasing order.
Core candidate and line-specific sequences were classified using LAST ver. 490 with a score of 272 corresponding to an E-value cut-off of 1E−100.
The percentages of intrinsic and partial genes in core candidate sequences (82 83%) were slightly higher than that in line-specific sequences (74 75%) (Supplementary Table S13).
Further, studies on core candidate markers for JE disease will characterize some more specific unknown facts about pathological mechanisms of marker regulation and expression.
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